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Diversité et activité des communautés microbiennes dans des sédiments marins associés aux émissions de fluides froids ArchiMer
Lazar, Cassandre Sara.
A large portion of the greenhouse gas methane on Earth is trapped in marine sediments. However, little methane actually reaches the hydrsphere and the atmosphere, because it is efficiently consumed by anaerobic methane-oxidizing Archaea (ANME). This methane is mainly biogenic in marine sédiments, and is produced by methanogenic Archaea. Methane ascends from deep sources to the seabed, in cold seep sites of continental margins. In order to study microbial community diversity in cold seeps, and to determine geochemical factors that control these microbial communities, we compared four geochemically and geologically distinct sites. In this work, molecular tools (PCR, RT-PCR, DGGE, cloning) as well as genetic markers (16S rRNA, mcrA, dsrB) were employed, to...
Tipo: Text Palavras-chave: Diversité moléculaire; Archaea; Méthanogène; ANME; McrA; DsrB; DGGE; Volcan de boue; Pockmark; Saumures; Fluides froids; Méthane; Mer de Norvège; Mer Méditerranée; Sédiments.; Molecular diversity; Archaea; Methanogen; ANME; McrA; DsrB; DGGE; Mud volcano; Pockmark; Brines; Cold seep; Methane; Norwegian Sea; Mediterranean Sea; Sediments..
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00031/14250/11534.pdf
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Diversité phylogénétique et fonctionnelle des communautés microbiennes incultivées des sédiments marins de la marge de Sonora, Bassin de Guaymas (Golfe de Californie) ArchiMer
Vigneron, Adrien.
At continental margins, and more particularly in cold seep areas, microbial and animal communities were locally detected at the surface of the sediments. These communities grow using reduced chemical compounds (H2S, Methane, COZ ...) contained in the percolated cold fluids and produced by both geological and microbial processes. ln order to study microbial community diversity in these ecosystems and their role in the environment as well as to understand the environmental factors influencing the distribution and ecophysiology of these communities, surface (0-20 cmbsf) but also deeper (<9 mbsf) sediments were collected at the Sonora Margin. Microbial communities have been studied using various molecular, cultural and microscopy approaches. This research...
Tipo: Text Palavras-chave: Fluides froids; Marge de Sonora; Bassin de Guaymas; Diversité moléculaire; Communautés microbiennes; Archaea; Bacteria; Méthanogènes; Methanogens; Microbial communities; Molecular diversity; Guaymas Basin; Sonora Margin; ANME; SRb; Methane; Sediments; Cold seeps.
Ano: 2012 URL: https://archimer.ifremer.fr/doc/00498/60956/64359.pdf
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Etude de la diversité métabolique des micro-organismes des sources hydrothermales ArchiMer
Byrne, Nathalie.
Since the discovery of oceanic hydrothermal vents, microbial diversity of this extreme environment was studied using both cultivation and molecular approaches. In this study the microbial diversity of active chimney from both TAG and Lucky Strike sites (Mid Atlantic Ridge) was investigated using both molecular survey and continuous enrichment culture in a bioreactor. To reproduce as close as possible environmental conditions the cultures were performed in oligotrophic conditions, in the presence of hydrogen and carbondioxide in the gas sweeping mix or using in situ sampled fluid/sea water mix as culture medium. The effect of environmental changes such as temperature and the absence or presence of sulphur was also investigated. Archaeal and Bacterial...
Tipo: Text Palavras-chave: Culture; Molecular diversity; Metabolism; Hydrothermal vents; Microorganisms; Microbiology; Culture; Diversité moléculaire; Métabolisme; Sources hydrothermales; Micro organismes; Microbiologie.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-7295.pdf
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